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Genesis 6:3

And God said: My spirit shall not remain in man for ever, because he is flesh, and his days shall be a hundred and twenty years.

Challoner: His days shall beThe meaning is, that man's days, which before the flood were usually 900 years, should now be reduced to 120 years. Or rather, that God would allow men this term of 120 years, for their repentance and conversion, before he would send the deluge.
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The verse in its languages

Vulgate (Clementine)
Dixitque Deus : Non permanebit spiritus meus in homine in æternum, quia caro est : eruntque dies illius centum viginti annorum.
Septuagint (Swete)
καὶ εἶπεν Κύριος ὁ θεός Οὐ μὴ καταμείνῃ τὸ πνεῦμά μου ἐν τοῖς ἀνθρώποις τούτοις εἰς τὸν αἰῶνα, διὰ τὸ εἶναι αὐτοὺς σάρκας· ἔσονται δὲ αἱ ἡμέραι αὐτῶν ἑκατὸν εἴκοσι ἔτη.
Hebrew (WLC)
וַיֹּ֣אמֶר יְהוָ֗ה לֹֽא יָד֨וֹן רוּחִ֤י בָֽאָדָם֙ לְעֹלָ֔ם בְּשַׁגַּ֖ם ה֣וּא בָשָׂ֑ר וְהָי֣וּ יָמָ֔יו מֵאָ֥ה וְעֶשְׂרִ֖ים שָׁנָֽה

Haydock's commentary

His days shall be, &c. The meaning is, that man's days, which before the flood were usually 900 years, should now be reduced to 120 years. Or rather, that God would allow men this term of 120 years, for their repentance and conversion, before he would send the deluge. (Challoner)

He spoke therefore to Noe in his 480th year. (St. Augustine) Those who suppose, that he foretold this event 20 years later, think with St. Jerome, that God retrenched 20 years from the time first assigned for penance. The Spirit of the sovereign Judge was fired with contending; or, as others translate it, with remaining quiet as in a scabbard, and bearing with the repeated crimes of men. He resolved to punish them severely in this world, that he might shew mercy to some of them hereafter. (St. Jerome, 9. Heb.) (Calmet)

If we suppose, that God here threatens to reduce the space of man's life to 120 years, we must say, at least, that he did it by degrees: for many lived several hundred years, even after the deluge. In the days of Moses, indeed, few exceeded that term. But we think the other interpretation is more literal, and that God bore with mankind the full time which he promised. (Worthington)

From Haydock's Catholic Bible Commentary, 1859, in the transcription made by Todd Easton and kept online by John Paul Wohlschied at johnblood.gitlab.io/haydock.

The Fathers on this verse

Clement of Alexandria The Stromata, or Miscellanies, Chapter XI. The Mystical Meanings in the Proportions of Numbers, Geometrical Ratios, and Music

Now the number 300 is, 3 by 100. Ten is allowed to be the perfect number. And 8 is the first cube, which is equality in all the dimensions, length, breadth, depth. “The days of men shall be,” it is said, “120 ( ρκ´ ) years.” And the sum is made up of the numbers from 1 to 15 added together. And the moon at 15 days is full.

Tertullian On Monogamy, Chapter I

Heretics do away with marriages; Psychics accumulate them. The former marry not even once; the latter not only once. What dost thou, Law of the Creator? Between alien eunuchs and thine own grooms, thou complainest as much of the over-obedience of thine own household as of the contempt of strangers. They who abuse thee, do thee equal hurt with them who use thee not. In fact, neither is such continence laudable because it is heretical, nor such licence defensible because it is psychical. The former is blasphemous, the latter wanton; the former destroys the God of marriages, the latter puts Him to the blush. Among us , however, whom the recognition of spiritual gifts entitles to be deservedly called Spiritual, continence is as religious as licence is modest; since both the one and the other are in harmony with the Creator. …

Origen Origen De Principiis, Chapter III

7. But if this is to be understood as spoken of the Spirit of God, since Adam also is found to have prophesied of some things, it may be taken not as of general application, but as confined to those who are saints. Finally, also, at the time of the flood, when all flesh had corrupted their way before God, it is recorded that God spoke thus, as of undeserving men and sinners: “My Spirit shall not abide with those men for ever, because they are flesh.” By which, it is clearly shown that the Spirit of God is taken away from all who are unworthy. In the Psalms also it is written: “Thou wilt take away their spirit, and they will die, and return to their earth. …

Origen Origen Against Celsus, Chapter XXXVIII

Since we hold that the great God is in essence simple, invisible, and incorporeal, Himself pure intelligence, or something transcending intelligence and existence, we can never say that God is apprehended by any other means than through the intelligence which is formed in His image, though now, in the words of Paul, “we see in a glass obscurely, but then face to face.” And if we use the expression “face to face,” let no one pervert its meaning; but let it be explained by this passage, “Beholding with open face the glory of the Lord, we are changed into the same image, from glory to glory,” which shows that we do not use the word in this connection to mean the visible face, but take it figuratively, in the same way as we have shown that the eyes, the ears, and the other parts of the body are employed. …

St Hippolytus On the Psalms, Section I

4. The number fifty, moreover, contains seven sevens, or a Sabbath of Sabbaths; and also over and above these full Sabbaths, a new beginning, in the eight, of a really new rest that remains above the Sabbaths. And let any one who is able, observe this (as it is carried out) in the Psalms with more, indeed, than human accuracy, so as to find out the reasons in each case, as we shall set them forth. Thus, for instance, it is not without a purpose that the eighth psalm has the inscription, “On the wine-presses,” as it comprehends the perfection of fruits in the eight; for the time for the enjoyment of the fruits of the true vine could not be before the eight. And again, the second psalm inscribed “On the wine-presses,” is the eightieth, containing another eighth number, viz., in the tenth multiple. …

St Augustine City of God, Utterances of the Prophet Isaiah Regarding the Resurrection of the Dead and the Retributive Judgment

But because he said, “Your heart shall rejoice,” lest we should suppose that the blessings of that Jerusalem are only spiritual, he adds, “And your bones shall rise up like a herb,” alluding to the resurrection of the body, and as it were supplying an omission he had made. For it will not take place when we have seen; but we shall see when it has taken place. For he had already spoken of the new heavens and the new earth, speaking repeatedly, and under many figures, of the things promised to the saints, and saying,“There shall be new heavens, and a new earth: and the former shall not be remembered nor come into mind; but they shall find in it gladness and exultation. Behold, I will make Jerusalem an exultation, and my people a joy. …

St Augustine Answer to the Letters of Petilian, the Donatist, Chapter 18

Chapter 18. 40. Petilianus said: "Nor is it, after all, so strange that you assume to yourself the name of bishop without authority. This is the true custom of the devil, to choose in preference a mode of deceiving by which he usurps to himself a word of holy meaning, as the apostle declares to us: ‘And no marvel,’ he says: ‘for Satan himself is transformed into an angel of light. Therefore it is no great thing if his ministers also be transformed as the ministers of righteousness.’ Nor is it therefore a marvel if you falsely call yourself a bishop. …

St John Chrysostom The Homilies of St. John Chrysostom on Paul's Epistle to the Romans, Homily XIII on Rom. vii. 14

What then? Are we, it will be said, to cut our bodies in pieces to please God, and to make our escape from the flesh? and would you have us be homicides, and so lead us to virtue? You see what inconsistencies are gendered by taking the words literally. For by “the flesh” in this passage, he does not mean the body, or the essence of the body, but that life which is fleshly and worldly, and uses self-indulgence and extravagance to the full, so making the entire man flesh. For as they that have the wings of the Spirit, make the body also spiritual, so do they who bound off from this, and are the slaves of the belly, and of pleasure, make the soul also flesh, not that they change the essence of it, but that they mar its noble birth. …

St John Chrysostom The Homilies of St. John Chrysostom on Timothy, Titus, and Philemon, 1 Timothy 4:11-14

Such as these the Scripture calls men without souls. “My Spirit (it is said) shall not always abide in these men, because they are flesh.” ( Gen. vi. 3 , Sept.) Yet they had a soul, but because it was dead in them, He calls them flesh. For as in the case of the virtuous, though they have a body, we say, “he is all soul, he is all spirit,” so the reverse is said of those who are otherwise. So Paul also said of those, who did not fulfill the works of the flesh, “Ye are not in the flesh.” ( Rom. viii. 9 .) Thus those who live in luxury are not in the soul or in the spirit.

St Jerome Against Jovinianus, Book II

15. So far I have dealt with the arguments and examples of philosophers. Now I will pass on to the beginning of the human race, that is, to the sphere which belongs to us. I will first point out that Adam received a command in paradise to abstain from one tree though he might eat the other fruit. The blessedness of paradise could not be consecrated without abstinence from food. So long as he fasted, he remained in paradise; he ate, and was cast out; he was no sooner cast out than he married a wife. While he fasted in paradise he continued a virgin: when he filled himself with food in the earth, he bound himself with the tie of marriage. …

St Jerome To Pammachius against John of Jerusalem

28. In the symbol of our faith and hope, which was delivered by the Apostles, and is not written with paper and ink, but on fleshy tables of the heart, after the confession of the Trinity and the unity of the Church, the whole symbol of Christian dogma concludes with the resurrection of the flesh. You dwell so exclusively upon the subject of the body, harping upon it in your discourse, repeating first the body, and secondly the body, and again the body, and nine times over the body, that you do not even once name the flesh; whereas they always speak of the flesh, but say nothing of the body. I would have you know that we see through what you craftily add, and with wise precaution seek to conceal. …

St Ambrose On the Mysteries, Chapter III. St. Ambrose points out that we must consider the divine presence and working in the water and the sacred ministers, and then brings forward many Old Testament figures of baptism

10. Take another testimony. All flesh was corrupt by its iniquities. “My Spirit,” says God, “shall not remain among men, because they are flesh.” Whereby God shows that the grace of the Spirit is turned away by carnal impurity and the pollution of grave sin. Upon which, God, willing to restore what was lacking, sent the flood and bade just Noah go up into the ark. And he, after having, as the flood was passing off, sent forth first a raven which did not return, sent forth a dove which is said to have returned with an olive twig. You see the water, you see the wood [of the ark], you see the dove, and do you hesitate as to the mystery?

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The Catechism cites this verse